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Abstract To investigate the epidemiology of Clostridium difficile infection in Romanian hospitals. Methods: A survey was conducted at nine hospitals throughout Romania between November and February Results: The survey identified patients with Clostridium difficile infection.

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The mean prevalence of Clostridium difficile infection was 5. The highest prevalences were Clostridium difficile infections were health care-associated in The origin was not determined in Clostridium difficile infection was severe in Polymerase chain reaction ribotype had the highest prevalence in all participating hospitals and represented Conclusion: The ribotype was the predominant cause of Clostridium difficile infections in Romania.

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In some specialized hospitals, the prevalence of Clostridium difficile infection was higher than the European mean prevalence, and this demonstrates the need for strict adherence to infection control programs.

Keywords: Clostridium difficile, epidemiology, ribotypeRomania The increasing incidence and severity of Clostridium difficile infections CDIs reported since in the United States and Canada 12 have been associated with the emergence of a hypervirulent ribotype 2.

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A decrease in ribotype infections in the United Kingdom has been reported following implementation of effective hospital infection control programs for CDI 5. The first confirmed cases of severe ribotype CDI in Romania occurred in However, very few data regarding the C. The present epidemiological survey aimed to evaluate the spread of CDI in patients admitted to selected hospitals throughout Romania and determine the epidemiology prevalence and the circulating ribotypes and the frequency of occurrence of severe C diff toxin cases.

Of the nine participating hospitals, seven are general hospitals; the others are specialized in infectious diseases H1 and gastroenterology H5respectively.

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The study data were collected, anonymized, and provided by the National Institute for Public Health.

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